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KMID : 1101220010330020237
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2001 Volume.33 No. 2 p.237 ~ p.244
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Effects of Squalene in the Skin of Aged Mouse : Molecularbiology and Ultrastructural Study
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Choi Wan-Soo
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Abstract
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To evaluate the effect of squalene (SQ) on the skin of the aged mouse, SQ was applied to the skin of the back of 10 month-aged ICR female mouse with 12 hour-interval for 10 days. Biochemical quantitative ana1ysis of total protein, the expression of epidermal growth factor (EGF) and histologic alterations in the SQ-treated skin using electron microscopes were performed. Total protein quantity was increased with the period of SQ treatment in SQ-treated group rather than in normal aged control group. The increment was especially remarkable on protein with 42kDa. In electron microscopic observation, there were only few germ cells in normal aged skin, however, the number of the cells started to increase after 3 days of SQ πeatment and were remarkable after 10 days. The differentiating cells in germ cell layers of SQ treated skin had relatively high electron-density of nucleus, and well developed RER, mitochondria, tonofilaments and free ribosome compared to normal aged Skin. The application of SQ to the skin of aged mouse could induce the increment of skin tissue total protein quantity and expression of EGF, and promote germ cell differentiation. Therefore, it is suggested that SQ plays a major role in differentiation or renewal of skin tissue of aged mouse.
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KEYWORD
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squalene, epidermal growth factor, electron microscopic, aged mouse
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